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The adenine nucleotide translocase ( ADP–ATP translocase), a transporter located in the inner mitochondrial membrane, transports ADP and ATP across the membrane. It is an antiporter. Recall that these adenine nucleotides are negatively charged: ADP3− and ATP4−.
Required:
a) Which direction is ADP3− transported during times of active oxidative phosphorylation?
b) What drives the transport of adenine nucleotides?
c) What is the ratio of ADP to ATP transported by the adenine nucleotide translocase? That is, how many ADP are transported for each ATP transported?
d) What drives the transport of H2PO4- across the membrane? Which direction is it transported during oxidative phosphorylation?

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Answer:

a) out of the mitochondrial matrix

b)the electrochemical gradient

c) 1 ADP+Pi = 1ATP thus, ratio is 1ADP:1ATP

d) H+ & H2PO4- is transported into the matrix in a process driven by the proton gradient.

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