Respuesta :
sa11 and pdh223 confer erythromycin resistance on f. johnsoniae, but not on e. coli found to be resistant to high levels of erythromycin
When pDH223 and pSA11 were introduced into F. Erythromycin resistance develops in Johnsoniae bacteria, but not in E. coli. The erythromycin resistance gene enables these altered cells to thrive when cultured on erythromycin-containing agar plates. On the erythromycin-containing nutritional medium in a petri plate, other cells that are not transformed by these two plasmids containing erythromycin resistance genes cannot grow. As a result, we are able to distinguish between transformed and untransformed cells. We will obtain erythromycin-resistant F-containing pure colonies of pDH223 and pSA11. cells from Johnsonia.
Both F. johnsoniae and E. coli may grow on nutritional medium containing erythromycin after receiving these two plasmids, pSA11 and pDH223, through the process ofof transformation. Both cells can grow and form colonies when sample one is placed on a petri dish with nutritional medium.
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