In order to add nucleotides to an expanding strand of RNA, RNA polymerase unwinds the double helix.
RNA Pol I: It synthesizes the pre-rRNA subunits that make up ribosomes, which is one of many forms of RNA Pol enzymes found in eukaryotes. RNA Pol II: It creates mRNA, snRNA, and microRNA precursors. RNA Pol III: It produces short RNAs, other rRNA precursors, and tRNA.
According to the DNA wrapping model of transcription, DNA bending and wrapping around RNA polymerase results in the DNA helix being unwound at the enzyme's catalytic site, which induces strand separation both before and during transcript elongation.
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